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Title: Evaluating Performance of Mung Bean Genotypes by Inoculating Seed with Bacterial Strains under Green House Conditions
Authors: Khalid Mehmood, Hamad Naeem, Hira Abbasi, Majid Mahmood Tahir, Muhammad Jamil
Journal: Journal of Agriculture and Veterinary Science
Publisher: Zoo Botanica (SMC Pvt.) Ltd.
Country: Pakistan
Year: 2023
Volume: 2
Issue: 1
Language: English
Keywords: Mung beanN-fixationRhizobiumPhosphate solubilizing bacteriaNodulation
Mung bean (Vigna radiata L.) is regarded as an ancient pulse crop of Asia and holds key position due to its high nutrient value. The reason for low yield of mung bean in Pakistan is poor soil and nutrient management and crop husbandry. The compatible strains of bacteria can enhance nitrogen fixation capacity and yield of the mung bean. The present study aimed to evaluate the response of three mung bean genotypes by inoculating with rhizobium and phosphat solubilizing bacteria under controlled conditions. The experiment comprised of three varieties that are (V1) AZRI MUNG-2006, (V2) NM-06, (V3) NCM-21 inoculated with bacterial strain having a control with no inoculam as (T0) Control, (T1) Rhizobium, (T3) Phosphate solubilizing bacteria, (T4) Rhizobium strain + Phosphate solubilizing bacteria. The bacterial treatments were inoculated with seeds of selected genotypes and grown in pots upto full vegetative stage. The data on different growth related parameters were taken to analyse the performance to bacterial strains on tested varieties. The investigations revealed that leaf area, cholorophyll content, no of pod/plant, no of nodules, nodules dry weight, root and shoot dry were significantly (P<0.05) influenced by bacterial strains in all the varieties. The rhizobium strain was found superior to attain highest root shoot dry weight probably due to maximum N fixation and supply to the plants that increased as leaf area, cholorphyll content and in turn produced maximum growth. The availability and uptake of nutrient is treated varieties is witnessed by increased aveages values of plant N (18.08 mg/kg) and P (2.23 mg/kg) in Rhizobium and PSB treated pots, respectively. The PSB singly or in comination with rhizobium also enhanced growth in all the varieties. We concluded the selection of suitable bacterial strains could help to improve nodulation efficiecy of legumes for sustainable plant growth and soil improvement. The study provided new avenues of research needed to be conducted under field conditions to select compatible strains for different varities for the area.
To evaluate the response of three mung bean genotypes by inoculating them with Rhizobium and phosphate solubilizing bacteria (PSB) under controlled greenhouse conditions, assessing their performance in terms of nodulation and nitrogen fixation.
The study involved three mung bean genotypes (AZRI MUNG-2006, NM-06, NCM-21) inoculated with four treatments: Control (no inoculum), Rhizobium (T1), Phosphate Solubilizing Bacteria (T2), and Rhizobium + Phosphate Solubilizing Bacteria (T3). Seeds were inoculated and grown in pots until the full vegetative stage. Data was collected on various growth parameters, including leaf area, chlorophyll content, number of pods per plant, number of nodules, nodule dry weight, root dry weight, shoot dry weight, and plant N and P content. Statistical analysis (LSD at P<0.05) was used to determine significant differences.
graph TD
A[Select 3 Mung Bean Genotypes] --> B[Inoculate Seeds];
B --> C[Apply Treatments: Control, Rhizobium, PSB, Rhizobium+PSB];
C --> D[Grow plants in pots under greenhouse conditions];
D --> E[Collect data on growth parameters];
E --> F[Analyze data statistically LSD];
F --> G[Evaluate performance of genotypes and treatments];
G --> H[Draw Conclusions];
The study highlights the potential of bacterial inoculants, particularly Rhizobium and PSB, to enhance mung bean growth and nutrient uptake. Rhizobium's role in nitrogen fixation and PSB's ability to solubilize phosphorus are crucial for improving plant nutrition and yield. The observed differences among genotypes suggest a genetic component to their response to bacterial inoculation. The findings support the use of biofertilizers for sustainable agriculture, improving soil fertility and reducing reliance on synthetic fertilizers.
Bacterial strains significantly influenced leaf area, chlorophyll content, number of pods per plant, number of nodules, nodule dry weight, root dry weight, and shoot dry weight. Rhizobium inoculation (T1) generally led to the highest root-shoot dry weight, likely due to enhanced nitrogen fixation. Phosphate solubilizing bacteria (PSB) alone or in combination with Rhizobium also improved growth. Variety NM-06 showed the highest leaf area and N content, while V2 showed the highest P content. Rhizobium inoculation resulted in a two-fold increase in plant N concentration, and PSB inoculation led to the highest plant P concentration.
The selection of suitable bacterial strains, such as Rhizobium and PSB, can significantly improve nodulation efficiency, nitrogen fixation, and overall growth of mung bean. This approach offers a sustainable method for enhancing legume productivity and soil health. Further research under field conditions is recommended to identify compatible strains for different varieties and regions.
1. Leaf Area: The study found that variety NM-06 showed the highest mean leaf area of 44.3 cm².
2. Plant Height: Treatment T1 (Rhizobium) resulted in the maximum plant height of 52.80 cm.
3. Plant N Content: Variety V2 showed the highest N content of 18.08 mg/kg.
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