DefinePK

Abstract

In this study, the enantiomeric separation of racemic phenylalanine using hydrophobic polyethersulfone and hydrophilic regenerated cellulose membranes was performed. BSA and lipase were used as chiral selector. The complexes of DL-PHE-chiral selector were ultrafiltrated by the stirred ultrafiltration cell. During the ultrafiltration, the samples taken from permeate were analyzed with liquid chromatography system using Chiral-pak AD-H column for the determination of D-PHE and L-PHE enantiomers. The effectiveness of chiral separation was char¬acterized in terms of the values of enantiomeric excess (ee%). Chemical structures and zeta potentials of membranes were determined by Fourier transforms infrared spectroscopy, and by an electrokinetic analyzer, respectively. BSA showed affinity to D-PHE, while lipase showed affinity to L-PHE. The higher enantiomeric excess was obtained using lipase chiral selector and PES membrane.