DefinePK

Abstract

Background. Tooth extraction is a procedure to remove teeth from inside the socket which can cause damage to the tooth socket area. Black Soldier Fly (BSF) (Hermetia illucent) contains as much as 35% chitin found in shedding and cocoons. Chitin can be converted to chitosan. Nanochitosan is the smallest part of chitosan. Chitosan has high osteoconductivity properties that promote bone regeneration. Demineralized Dentin Matrix (DDM) came from human teeth that have been extracted is an organic material obtained from dentin that has osteogenic capabilities. Objective. To determine the potential of the combination of nanochitosan BSF pupae and DDM in forming RANKL, and OPG after tooth extraction. Metode. The left mandibular incisive tooth guinea pig is extracted, then the socket is filled with a Polyethylene glycol gel as a placebo in the control group (n=9), and the treatment group (n=9) is filled with a combination of nanochitosan BSF pupae and DDM from human teeth gel. The gel is inserted into the socket until it is full then suture with non-absorbable silk. The guinea pig in euthanasia saw the expression of RANKL, and OPG on the 7th, 14th, and 21st days. The RANKL, OPG, and Osteocalcin research results using Oneway ANOVA (p<0.05).Result. There is a significant difference between the treatment and control group with the significance value in the expression RANKL, OPG (0.000) (p<0.05). Conclusion: Combination of nanochitosan BSF pupae and DDM from human teeth has an effect in increasing the expression of Osteoprotegerin (OPG) and decreasing RANKL on the -7th, -14th, and -21st days and might help increase bone remodeling.