DefinePK

Abstract

Aim of research: This research focus on defining the immune environment of amoebiasis through whereas IL-8 and IL-25 cytokines affect host defenses toward Entamoeba histolytica infection .Materials and Methods: The study involved 44 confirmed patients together with 44 healthy controls as subjects during a 5-month research period in multiple healthcare facilities between October 2024 and February 2025. Structured interviews alongside medical record examinations produced sociodemographic data which enabled ELISA-based blood analysis of cytokines .Results: The study results indicated that IL-8 concentrations were notably increased among patients at 223.34 pg/mL while controls maintained levels at 82.51 pg/mL according to statistical analysis (p=0.001). The research data demonstrated increased IL-25 PG/mL levels in patients at 1107.97 pg/mL which was higher than the levels observed in controls at 496.70 pg/mL thus demonstrating shared importance of these cytokines in the immune response to infection .Conclusion: The examined patient population demonstrates acute inflammation through elevated IL-8 measurements and demonstrates adaptive immunity through elevated IL-25 levels while infected with E. histolytica. The discovered elevated levels of cytokines confirm that they function as vital biomarkers to determine disease severity and warrant additional investigation to enhance knowledge about the paths amoebiasis uses to interact with human hosts.