DefinePK

Abstract

Gallstone disease (GSD) is a prevalent and painful disorder characterized by the development of solid deposits in the gallbladder or bile ducts, including cholesterol, bile pigments, and calcium. While obesity is the main risk factor for GSD, the condition can also affect individuals who are not obese. This study examined the factors in obese and non-obese patients that may contribute to the formation and progression of gallstones, emphasizing the roles of β-Hydroxy β-Methylglutaryl Co-A gene expression and Spexin levels in driving inflammation.
The study involved 120 participants: 30 patients with symptomatic gallstones and obesity, 30 patients with symptomatic gallstones but no obesity, 30 obese patients without gallstones, and 30 healthy controls. Lipid profile levels (total cholesterol, triglycerides, HDL, LDL, and VLDL) were measured using an automatic Fuji film analyzer. Serum C-reactive protein (CRP) was quantified using an I-Chroma reader. Serum SPX levels were determined by enzyme-linked immunosorbent assay (ELISA), and HMG Co-A gene expression was analyzed using quantitative polymerase chain reaction (qPCR). Data were analyzed with SPSS version 28, with statistical significance set at P < 0.05.
The findings indicated an increase in lipid profile values and a decrease in HDL levels in all patient groups compared to controls, which was associated with heightened inflammation, as reflected by elevated CRP levels. Additionally, there were decreased SPX levels and a rise in HMG Co-A gene expression (P < 0.05).
In conclusion, the study suggests that the levels of SPX are negatively correlated with HMG Co-A gene expression, which may play a crucial role in triggering inflammation in gallstone patients, thus contributing to the progression of the disease, particularly in obese individuals.