DefinePK

Abstract

Introduction: Lung cancer is the major type of cancer that is developing all over the world. Approximately 1.8million new people were affected by lung cancer in 2012 among which 1.6million people were fatal. Kigelia pinnata is also known as balloon vine, climber plant. It belongs to order sapindaceae and it has a high medicinal value.
AIM: To assess the anticancer activity of Kigelia pinnata against human A549 lung cancer cell line.
Materials and method: The anticancer effect of kigelia pinnata on A549 was measured by MTT assay and the cell morphological evaluation done by inverted phase contrast microscopy.
Results and Discussion: From the MTT assay plant extract of Kigelia pinnata showed approximately 50% cell inhibition at 30µl/ mg concentration after 24hrs of treatment. Further it was confirmed with morphological evaluation using phase contrast microscopy. Overall, the MTT result clearly shows that the K.pinnata has significantly reduced the cell viability in dose dependent manner effect in cell proliferation.
Conclusion: From the results, the extracts were cytotoxic to the lung cancer cells at 30µl/ mg concentration and taken as (IC50,µl/ mg) value for further experiments. However more research is needed to understand the mechanisms of cytotoxicity of the plants.