DefinePK

Research Objective

To document a new host association for the encyrtid wasp, Psyllaphycus diaphorinae, with the olive psyllid, Euphyllura olivina, in Pakistan.

Methodology

Parasitized nymphs of Euphyllura olivina were collected from olive trees in the Pothwar region of Pakistan (districts Chakwal and Rawalpindi) between 2017 and 2020. Mummified psyllids were collected using a camel hairbrush and transferred to petri plates. Adult psyllids and infested leaves were also collected. Emerged parasitoids were collected, mounted, and identified using stereomicroscopes and slide microscopes, referencing taxonomic literature. Images were prepared using specialized software, and a distribution map was created using ArcGIS.

Methodology Flowchart

                        graph TD
    A[Collect parasitized Euphyllura olivina nymphs] --> B[Visit olive growing areas in Pothwar region];
    B --> C[Collect mummified psyllids and infested leaves];
    C --> D[Transfer to laboratory for rearing];
    D --> E[Collect emerged parasitoids];
    E --> F[Identify psyllids and parasitoids];
    F --> G[Prepare digital images of specimens];
    G --> H[Create distribution map];
    H --> I[Document new host association];                    

Discussion

The discovery of Euphyllura olivina as a new host for Psyllaphycus diaphorinae expands the known host range of this parasitoid and contributes to a better understanding of the psyllid fauna in Pakistan. This finding is significant for potential biological control strategies.

Key Findings

The encyrtid wasp, Psyllaphycus diaphorinae, was reared from nymphs of Euphyllura olivina, an olive psyllid. This represents a new host association for P. diaphorinae, which was previously known to be associated with Diaphorina citri. The study provides a distribution map with new localities for the parasitoid and its host in Pakistan.

Conclusion

This study establishes Euphyllura olivina as a new host for Psyllaphycus diaphorinae in Pakistan, specifically in the Pothwar region, thereby extending the known host association and geographical distribution of this parasitoid.

Fact Check

- The study reports a new host record for Psyllaphycus diaphorinae from Pakistan.
- Euphyllura olivina is identified as the new host psyllid.
- Collections were made in the Pothwar region of Pakistan, specifically in districts Chakwal and Rawalpindi, during the years 2017-2020.

Mind Map