DefinePK

Abstract

Selenium nanoparticles (SeNPs) are tiny particulates, which possess the ability for assimilation by biological systems, and have garnered attraction among researchers due to its exceptional biological properties and potential therapeutic functionalities. In the present study, SeNPs were synthesized via an effective and eco-friendly technique using leaf extract of Mukia maderaspatana. Then SeNPs were characterized by various analytical techniques. UV-Vis spectroscopy showed absorption peak at 265 nm. Biomolecules acts as stabilizers for synthesized SeNPs. Alcoholic group, nitro compounds, aromatic compounds in SeNPs is revealed by the O-H bond, N-O bond and C-C bond by FTIR analysis. SEM analysis showed dimensions of synthesized SeNPs ranging from 73.24 nm to 187.6 nm. EDX analysis highlights the elemental composition of SeNPs as (13.75%) of Selenium, (69.27%) of oxygen and (16.98%) of sodium, and XRD spectroscopy revealed the crystalline structure of SeNPs. The antioxidant potential of SeNPs via in vitro assays like NO, ABTS, FRAP and H2O2 showed higher inhibition as 86.71%, 89.18%, 87.54%, and 89.9% at a concentration of 50 µg/ml. Anti-inflammatory effects of SeNPs demonstrated a noteworthy inhibition of Bovine Serum Albumin, Egg Albumin denaturation as 78% and 77%, also in membrane stabilization with increase in the concentrations of SeNPs it stabilizes the cellular membranes. Brine shrimp lethality assay performed to assess the cytotoxicity effects of SeNPs and recorded the 50% of survival at 80 µg/mL of concentration, and thereby validated its biosafety profile and potential therapeutic index. Collectively, this investigation highlights the multifunctional capabilities of SeNPs as promising candidates for applications in the biomedical field.