DefinePK

Abstract

Many genes are mutated in acute myeloid leukemia patients, one of them is Met gene. the aim study was to study the single nucleotide polymorphisms in one loci of met gene in a sample of Iraqi patients. We diagnosed the polymorphism and compared the results with heathy volunteers’ gene. A set of forward and reverse primers were used to detect and amplificated met gene by PCR technique. The allele frequency then detected by Restriction fragment length polymorphism (RFLP) for patients and control healthy groups. Non-significant results were found in the recurrence of homozygous AA allele of met gene in AML patients which was 75.67 % while in healthy volunteers was 86%, but the heterozygotes AG of met gene was 24.32 % in AML patients and 14 % in healthy group. The finding of our study show that heterozygotes AG is more related with patients than control group and it occurs more frequently in female patients than male. while homozygous AA appears more frequent in healthy control than the patients and more frequent in male than female.