DefinePK

Abstract

Due to its cost-effectiveness and eco-friendliness, the production of silver nanoparticles (AgNPs) utilizing biological agents has attracted a lot of attention. In this study, we investigate the potential of the Serratia marcescens W2 strain as a bio-factory for the production of silver nanoparticles and evaluate the biocontrol capability of this strain against fungal phytopathogens as well as its impact on wheat seeds. Using the extracellular enzymes and metabolites produced by Serratia marcescens W2, AgNPs were biosynthesised. The size, form, and composition of the AgNPs were determined using a variety of analytical techniques, such as X-ray diffraction (XRD), and scanning electron microscopy (SEM). AgNPs' impressive ability to inhibit fungal growth in vitro experiments demonstrates their robust biocontrol capabilities. Microscopic and biochemical investigations helped to better clarify the AgNPs' mode of action against these phytopathogens. Additionally, investigations on seed germination and seedling growth were used to evaluate the effect of the AgNPs on wheat seeds. As a result of the application of AgNPs, seed germination rates, and seedling vigor dramatically increased, according to the findings. Additionally, compared to the control group, the seedlings treated with AgNPs showed enhanced resistance to fungal infection. Overall, the results of this study demonstrate the potential of Serratia marcescens W2 strain in the green synthesis of AgNPs with improved antifungal activities. Furthermore, the use of these AgNPs promotes the germination and growth of wheat seedlings, indicating their potential use as a biocontrol agent and seed treatment to safeguard crops against fungus phytopathogens in sustainable agriculture. To completely understand the underlying mechanisms and determine the long-term impacts of AgNPs on the ecosystem and human health, more research is necessary.