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Title: COMPARATIVE MICROBIAL EXAMINATION OF HOUSEHOLD DRINKING WATER AND WATER FILTRATION
Authors: Memoona Siddiqua, Sabeen Sabri, Mukarram Sharif
Journal: LGU Journal of life Sciences (LGUJLS)
Publisher: Lahore Garrison University, Lahore
Country: Pakistan
Year: 2025
Volume: 9
Issue: 1
Language: en
DOI: 10.54692/lgujls.2025.0901389
Keywords: Escherichia coliColiformsDrinking water,Microbiological contamination,spread plate method
Water is an essential element for survival on earth. Poor water quality is one of Pakistan's biggest health concerns and environmental issues. Drinking water quality has become worsedue to microbial and chemical contamination in water supplies and groundwater. To evaluate contamination, the standard plate count method, spread plate method, most portable number (MPN), and kit method were used. In the spread plate method, a specific amount of sample was poured and spread by the sterilized spreader evenly on the agar medium. The petri plates were incubated at 37° Celsius for 24 hours. After 24 hours, colonies were enumerated with the help of the colony counter. In the kit method, the sample water inoculated in the reagent vile showed characteristics of color if it was contaminated with coliforms. 70.36% of the household samples and 28.35% of fifiltered water samples were found with total coliform bacterial contaminated ions when compared with National Standards for Drinking Water Quality. In contrast to the Nestle mineral water, boiling water did not uncover any coliform bacterial colonies or other microorganisms. Hard and cloudy drinking water was encountered. Regulatory agencies must carefully examine drinking water sources for microbial contamination to prevent dangers to the public's health.
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