DefinePK

Abstract

The egg yolks play a significant role in preventing temperature related damages to the sperm’s acrosome and plasma membrane during cryopreservation. The present study aims to evaluate various egg yolk extenders in relation to quality indicators of post-thawed semen from Tapri goat bucks. Thus, total 60 ejaculates (n= 15/ animal) were collected from four healthy Tapri goat bucks entitled with A, B, C, and D. Semen samples were evaluated macro and microscopically and pooled in two groups viz; A and B. While preparing the Tris-based egg yolk extender; the egg yolk from duck was used in group “A” and Fayoumi chicken in group “B”. The diluted semen was packed in two different color straws (French straws) of 0.25 ml size, equilibrated for 2 hours at 5oC then frozen deep in the liquid nitrogen (at -196°C). The thawing of straws was attained at 37oC for 20 sec. The percentages of post-thawed total sperm motility, viability, normal morphology, and acrosomal membrane integrity were found significantly (p<0.05) better in group A (46.7±67, 49.33±1.397, 48.87±1.028, 50.60±1.302), then group B (42.67±0.6738, 43.73±0.9394, 44.73±1.112, 47±00), respectively. In conclusion, the extender containing duck egg yolk demonstrated significantly better (P<0.05) post-thaw quality parameters for Tapri goat bucks semen compared to that of fayoumi chicken.