DefinePK

Abstract

The pathogens Escherichia coli and Klebsiella pneumoniae are often involved in urinary tract infections (UTIs), which are common, especially in children. Due to their association with antibiotic resistance, which makes treatment tactics more difficult, the presence of the bla OXA-48 and bla IMP genes in these bacteria is essential. To detect two resistant genes, bla OXA-48 and bla IMP, in Klebsiella pneumoniae and Escherichia coli and to detect the variant genes by sequencing for each bacterium. In all, one hundred urine samples from children with UTIs were investigated. Using molecular techniques and gene sequencing, the isolates were tested for antibiotic resistance, ESBL phenotypic detection, and bla OXA-48 and bla IMP gene identification. The SPSS software was used to do the statistical analysis. K. pneumoniae and E. coli, the two most common isolates, exhibit significant rates of resistance to commonly used antibiotics. Genesbla OXA-48 was present in 92.3% of E. coli isolates and 100% of K. pneumoniae isolates, and bla IMP was present in 43.1% of E. coli isolates and 25.7% of K. pneumoniae isolates. The amplified blaIMP-1 sequences of E. coli and K. pneumoniae did not show any nucleic acid variation in the samples under analysis. While the results of the alignment of the amplified bla OXA samples with K.pneumoniaeand E. coli revealed the existence of two nucleic acid variations referring to reference nucleic acid sequences of K. pneumoniae and E. coli, respectively. The study shows that K. pneumoniae and E. coli both have significant levels of the bla OXA-48 and bla IMP genes. To prevent the spread of bacteria that cause antibiotic-resistant UTIs in children’s populations, our results emphasise the need for increased surveillance and monitoring of antibiotic regimens.