DefinePK

Abstract

Introduction: Silver nanoparticles (AgNPs) have gained attention for their antimicrobial properties, with plant-based synthesis emerging as a sustainable alternative to chemical methods. Africa montana, known for its medicinal properties, contains bioactive compounds that can facilitate nanoparticle formation. This study aims to synthesize AgNPs using Africa montana extract and evaluate their antimicrobial potential.
Materials and Methods: Africa montana leaves were collected, dried, and extracted in distilled water. The extract was mixed with silver nitrate (AgNO₃) solution, and nanoparticle formation was indicated by a color change. The AgNPs were characterized using UV-Vis Spectroscopy (for confirmation), SEM & TEM (for morphology and size), XRD (for structure), and EDX (for elemental composition). Antimicrobial activity was tested against Escherichia coli and Staphylococcus aureus using the disc diffusion method.
Results: The reaction mixture turned brown, confirming AgNP formation. UV-Vis spectroscopy showed an absorption peak at ~420 nm. SEM and TEM revealed spherical nanoparticles (20-30 nm). XRD confirmed crystallinity, while EDX detected silver presence. The AgNPs exhibited significant antibacterial activity against both bacterial strains.
Conclusion: This study successfully synthesized AgNPs using Africa montana, demonstrating an eco-friendly approach. The nanoparticles exhibited strong antimicrobial properties, suggesting potential applications in biomedical and environmental fields. Further research can explore their use in wound healing and drug delivery.