DefinePK

Abstract

The purpose of this research was to create and assess a nanoemulsion of grapeseed oil and Efavirenz to improve the drug's solubility in the formulation and, therefore, its bioavailability.Multiple components were tested in sample formulations to choose the best one based on composition, factorial design, clarity, and simplicity of nanoemulsion generation. Transcutol P was chosen as a co-emulsifying agent, tween 80 as the preferable emulsifying agent, and grapeseed oil as the oil.Factorial design was used to find an optimized formulation using the Design Expert software. To make a clear nanoemulsion, the components were combined, efavirenz was added, and a small amount of water was added to the mixture. Particle-size, Zeta-potential, TEM, DSC, TGA, In-vitro drug release and viscosity tests were all examined for the formulation. The normal droplet size of the formulation were found within the 10nm.The zeta-potential of the preparation was originated to be -20.1mV, representing that the formulation is rather stable. Centrifuge method was used to assess the efficiency of the drug entrapment. The In-vitro release studies of liquid formulation showed an increase in drug release profile of 75.38±1.31% in contrast to the marketed formulation (SUSTIVA600) tablet which showed the maximum release of 44.09±0.45%. The kinetics models were applied to the percentage release profile of the NE and it shows that the optimized formulation follows
the zero order showing constant release pattern. The stability studies for the optimized formulation were performed for in total 90 days in and the shelf life for the formulation was found to be 591 days. Nanoemulsions are the great carrier for improving the bioavailability and solubility of the any lipophilic drug which ultimately helps in increasing the potency of any formulation. Nanoemulsions are great choice for vaccine development and can further used in many other applications like in cosmetics, food and nutrition etc.