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Abstract

ABSTRACT Recent advancements in tissue engineering suggest that biomaterials, particularly decellularized extracellular matrix (ECM), have the potential to improve the targeting and effectiveness of regenerative therapies in the central nervous system. In present experimental study utilized powder derived from a decellularized rat brain tissue scaffold combined with a growth factor. PC12 cells were exposed to nerve growth factor (NGF) and dECM in a two-dimensional cell culture. Cell proliferation rate and percentage of viable cells were evaluated using trypan blue, while samples were stained with DAPI and cresyl violet. The mRNA expression patterns associated with nerve cell differentiation post-extraction were measured using the qRT-PCR method, and cell differentiation was assessed through immunocytochemical evaluation. The findings revealed that the differentiated PC12 cells exhibited a higher level of mRNA compared to the control cells. A statistically significant increase in mRNA level was observed for Nestin and β-tubulin III. Furthermore, the immunocytochemistry results showed that the differentiated PC12 cells expressed a higher level of these proteins compared to the control cells. The evaluation of neuronal differentiation induction in PC12 cells in two-dimensional conditions revealed a significant increase in the expression of neural differentiation biomarkers Nestin and β-Tubulin III in the treatment groups compared to the control group. This suggests that the combined effect of decellularized rat brain tissue powder and NGF can be considered an inducer of neural differentiation in PC12 cells.