DefinePK

Abstract

ABSTRACT The aim of this study was to understand the infection status and full sequence characteristics of porcine parvovirus type 4 (PPV4) in pig herds in a breeding farm in north western Sichuan, China. Two hundred ninety-six serum samples from the breeding farm were collected for testing, and the PPV4-specific fragments were amplified using PCR. The PCR products identified as positive were subjected to whole gene sequencing and genetic variation analysis. The results showed that the PPV4 positivity rate was 0.68% (2/296) in the collected samples. Sequence analysis of one of the strains, PPV4-MY, revealed some mutations in its whole genome, with a total of 24 base mutations and 6 amino acid mutations in the open reading frame, among which ORF2 and ORF3 were more variable than ORF1. Phylogenetic tree analysis revealed that PPV4-MY was most closely related to the strains detected in Shandong and Korea.