DefinePK

Abstract

ABSTRACT Nanotechnology has numerous applications in medicine, diagnostics, and science. Over the last ten years, nanotechnology has significantly influenced reproductive techniques such as in vitro maturation, fertilization, and follicular oocyte culture. Selenium nanoparticles have substantial applications in numerous fields including disease prevention and treatment, mammary glands’ development, reproduction, and immune systems. In present study, in vitro trial was performed to examine the effect of selenium nanoparticles (SeNPs) on mice embryo growth and development. For this purposes, sixty (n= 60) female mice were divided randomly into three equal groups (n= 20) including control and two treatment groups. The treatment groups received SeNPs at doses of 5 and 25 µg/ml, respectively. During the experiment period, mice embryo growth and development, cleavage rate, and blastocyst rate were analyzed. After the collection of oocytes from female mice, insemination and in vitro fertilization were done to culture embryo through in vitro study. It was found that during the embryonic stages, the treated group showed no significant (P>0.05) differences compared to control group. Moreover, the blastocyst rate exhibited no notable differences. Whereas, the cleavage rate was significantly (P<0.05) decreased compare with control group. Altogether, the current study showed that exposing mice embryos to SeNPs had detrimental effects on the cleavage rate in in vitro trial.