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Abstract

Background and Objective Although some investigations have previously been reported with this plant, the seed of the plant was not studied yet to date. This present study aimed to investigate in vivo and in vitro bioactivities of Diospyros blancoi. Cold methanolic extract of D. blancoi has been considered for evaluation of therapeutic activities such as analgesic, thrombolytic activity and anti-inflammatory. Materials and Methods Analgesic activity was tested by formalin induced licking and biting in mice, thrombolytic activity was carried out by using human blood sample and the anti-inflammatory effect was tested by carrageenan induced paw edema method. The seeds of D. blancoi was extracted with methanol. Results The extracts were used for the observation of analgesic activity, thrombolytic activity and anti-inflammatory activity. The D. blancoi had showed dose dependent significant analgesic activity in swiss albino mice in formalin induced method. They produced a protection of 55.67 and 73.47 from extract 100, 200 mg kg1501, respectively. In thrombolytic activity the extract shown 38.17 of clot lysis from 10 mg mL1501, in anti-inflammatory test they produced an inhibition of 24.81 and 27.23 at last hour. from extract 200 and 400 mg kg1501, respectively. Conclusion The results demonstrated that the methanol extract of D. blancoi seed had the moderate analgesic, anti- inflammatory and thrombolytic properties.