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Abstract

Background and Objectives Multi-drug resistant bacteria MDRB have become a widespread serious problem in tropical and subtropical countries. The present study was aimed to investigate the isolation, identification, antibacterial resistancesensitivity pattern of MDRB S. aureus, E. coli and S. pneumoniae from clinical samples and green synthesis of AgNPs for their control. Materials and Methods The routine isolation and identification steps were followed for MDRB from samples. The antibacterial resistantsensitivity pattern of isolated bacteria were tested against commercial antibiotics. In addition, Cippadessa baccifera plant mediated silver nano-particles were synthesized and tested against the target bacteria. The green synthesized silver nano-particles were characterized by using UV-Vis, XRD, EDX, FTIR and SEM analysis. Results About 136 bacterial isolates S. aureus 65, E. coli 60 and S. pneumoniae 11 were identified based on their colony morphology and microscopic observation. A total of 121 bacterial isolates were confirmed as resistant against selected antibiotics like penicillin, methicillin, ampicillin and amoxicillin clanonic acid. Plant mediated AgNPs exhibited strong antibacterial activity against target bacteria with better growth inhibition zones i.e., E. coli 350.9 mm, S. aureus 33.60.4 mm and S. pneumoniae 23.30.5 mm than other extracts. UV-Vis spectroscopy results of green AgNPs showed maximum absorption peak at 370 nm. XRD and SEM analysis revealed that AgNPs were face-centered, cubic structure being spherical in shape with an average particle size of 45 nm. FTIR result of nanoparticles showed capped with C-X, -CH3, NO, CN, CO functional groups of plant compounds. The particle size of AgNPs was found to be poly-dispersed nature. Conclusion The outcome of results suggested that, plant mediated AgNPs express better antibacterial effect against multi drug resistant bacteria MDRB and it can be used as a potential source for various biomedical applications.