DefinePK

Abstract

Background and Objective The advocacy for the alternative use of some combined therapy in herbal medicine is currently gaining more grounds in Nigeria than before due to incessant drug resistance phenomenon. Thus, the study was focused on the evaluation of the antibacterial property of the combined extract from Kalanchoe pinnata and Citrus aurantifolia for the inhibition of the otitis-media isolates. Materials and Methods The test organisms were isolated from otitis-media samples by standard bacteriological methods and identified by numerical taxonomic procedures using Jaccard Simple Matching Coefficient tool. The Kirby-Bauer disk diffusion susceptibility method was adopted for their sensitivity to varied concentrations of the combined extract. The obtained data were considered significant at p0.05 by using statistical package for social sciences SPSS, version 15 for paired-t-test correlation analysis. Results The predominant recovered isolates were Staphylococcus aureus 86.7, Streptococcus pneumoniae 60, Streptococcus pyogenes 80 and Proteus mirabilis 56.7. Relatively, the combined extract had greater antibacterial action especially on the Gram-ve Proteus mirabilis 119.30.14 than the single extract 71.50.09 and demonstrated above 70 sensitivity in line with the most sensitive reference antibiotics Ampiclox, Gentamicin and Ciprofloxacin at a significant level of p0.05. There was significant dose-dependent efficacy of the extract on these isolates. The lower concentration 10 g, significantly p0.05 exhibited stronger inhibitory actions 82.70.11-119.30.14 than the higher 30 g concentration which achieved a range of 71.90.07-99.30.10 inhibition against the test isolates. Conclusion The observed results indicated the strong antibacterial potential of the combined test extract against otitis-media pathogens and these will be supportive to scientific documentation for pharmacological utilization.