DefinePK

Abstract

Background and Objective Two new biphenyls, 3'-hydroxy-5-methoxy-3,4-methylenedioxybiphenyl 1 And 3'-hydroxy-5,5'-dimethoxy-3,4-methylenedioxybiphenyl 2 Have been isolated from Streptomyces sp. BO07, an endophyte in the root tissue of Boesenbergia rotunda L. Mansf A. Although, there are many biphenyls on anti-inflammatory activity have been reported. This study investigated the anti-inflammatory property of these biphenyls on LPS-induced macrophage RAW 264.7 cells. Materials and Methods The effects of the two biphenyl compounds were investigated on the formation of NO, PGE2, TNF-, IL-1, IL-6, IL-10 and also on inducible nitric oxide synthase iNOS and cyclo-oxygenase-2 COX-2 in LPS-induced murine macrophage RAW 264.7 cells. Results The data obtained were consistent with the modulation of TNF-, IL-1, IL-6, IL-10 production by these biphenyls at concentration of 5-20 g mL1501. A similar effect was also observed when LPS-induced NO release, iNOS and COX-2 enzyme expression were tested. The inhibitory effects were shown in concentration-dependent manners. However these compounds did not significantly reduce the formation of PGE2. Conclusion From the obtained results, it is concluded that these biphenyls possess anti-inflammatory activity on LPS-induced RAW 264.7 cells.