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Abstract

Background and Objective The neuroendocrine system is a variety of nervous and endocrine tissues distributed throughout the body either organized or associated with other organs. The present study aimed to identify and characterize the features of pinealocytes as one of these neuroendocrine cells NECs in order to establish a histological scheme. Materials and Methods A total of 40 adult apparently healthy New Zealand rabbits of both sexes were used. Pineal glands were obtained and subjected to the following reactions general, specific, highly specific and immunohistochemical reactions for neuroendocrine cells in addition to the ultrastructural examinations. Results The two types of pinealocytes Pinealocytes type I and II were contained electron dense secretory granules. These granules were reacted positively with Grimelius silver impregnation, Lead Hematoxylin and OFG stains. The pinealocytes showed moderate reactions with Gomori's aldehyde fuchsin and aldehyde thionine stains. By using of Gomori's chrome alum hematoxylin and performic acid alcian blue stains, the cytoplasmic granules showed intense reactions. Immunohistochemically, the pinealocytes were subjected to anti-CgA antibody, anti-NSE antibody and anti-melatonin antibody gave positive reactions. Conclusion It can be concluded that a model design for histological scheme that could be beneficial for biologists especially histologists in identification and characterization of neuroendocrine cells distributed throughout the body.