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Title: Anti-fungal Potential and Brine Shrimp Lethality Assay of in vitro Raised Clones of Celastrus paniculatus
Authors: Anusha Tharayil Koonapra Sasidharan, Kothanam Kuziyil Elyas
Journal: Asian journal of biological sciences
Year: 2019
Volume: 12
Issue: 4
Language: en
DOI: 10.10.17311/ajbs.2019.877.883
Background and Objective Celastrus panicultus Willd Celastraceae a climbing shrub is well known for its ability to improve memory. Because of its high therapeutic application, it has been over exploited and is now considered as endangered species. The present investigation was focused the anti-fungal potential and brine shrimp lethality assay of leaf extracts of in vitro raised clones with that of their mother plant. Materials and Methods The powdered leaves were extracted sequentially using a soxhlet apparatus with solvents such as petroleum ether, chloroform and methanol for 24 h. The extracts of both mother plant and in vitro raised clones were tested for anti-fungal assay and brine shrimp lethality assay. Results The results showed that the methanolic extract of both mother plant and in vitro raised clones were equally and effectively inhibited Phytophthora capsici and Rhizoctonia solani completely. The chloroform and methanol extract showed maximum mortality against Artemia salina. Conclusion The study revealed the anti-fungal activity and non-toxic nature of the leaf extracts of both mother plant and in vitro raised clones of C. paniculatus.
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