DefinePK

Abstract

Background and Objective Staphylococci strains causing bovine mastitis, has produced serious therapeutic problems. However, the underlying mechanisms are poorly understood and it is necessary to develop new effective method for accurate diagnosis. Resazurin micro-plate method was used for the diagnosis of bovine mastitis caused by staphylococci. The objective of this study was to discuss about the method of resazurin micro-plate for the diagnosing of bovine mastitis which caused by staphylococci. Methodology While for testing the infected mastitis milk samples were inoculated in the selection of the Staphylococcus-mBP medium with 96-well micro-plate and pure cultures of bacteria and after 2 h of the incubation the resazurin indicator was added to that solution up to 0.4 g L1501 and then continued the cultivation for 10 h, depending on whether the medium color changes of bacterial growth. For statistical analysis chi-square test was performed using SPSS Software version 22 IBM SPSS Statistics for Windows, Armonk, NY, USA IBM Corp.. Results The results from the bacterial pure culture plate showed that the resazurin micro-plate indicated the growth of S. aureus i.e., S. epidermidis, S. chromogens, S. haemolyticus, S. simulans , S. xylosus and E. coli did not appear on the culture plates. Further from the investigated results showed that the color changing in the medium was due to the staphylococcal concentration and time incubation. However, the results of the infection milk samples showed that the resazurin micro-plate had a detection sensitivity of 1103 CFU mL1501 and in 12 h the diagnosis could be obtained. Conclusion Furthermore from the results of the mastitis milk samples, the positive culture plate showed that the resazurin micro-diagnosis of S. aureus-positive and negative milk sample at the rate of 98.3 n 60 and 95.0 n 40 respectively. Similarly, from all the investigated results it is concluded that the resazurin micro-plate can replace the conventional method for rapid diagnosis of Staphylococcus milk samples.