DefinePK

Abstract

This study aimed to investigate the normality and rate of acrosome, sperm head, mid-piece and sperm tail by using the Spermac staining technique. Semen from 10 males of Arabian sand gazelle was collected under anesthesia by electro-ejaculator once a month for 12 months during 2009. The morphological features acrosome, head, mid-piece and tail and total morphological defect were investigated by using Spermac stain technique under light microscope. The results showed that the mean of total morphological defects rate was 22.202.62, while the acrosomal defect rate was 12.302.55. The most observed acrosomal defect types were swollen, missed and dissolved acrosome. The most frequently defect seen were microcephalic, mid-piece defect, coiled tail and rarely double tails. There were considerable reduction in the number of sperm abnormalities. It was concluded at the end of this study the Spermac staining technique could provide a detailed observation for gazelle spermatozoa, especially the acrosomal region and could be employed in the determination of all types of defects.