DefinePK

Abstract

In addition to the morphological, skeletal and biometric characteristics, karyological study is the key to the animal identification and classification. Due to the insufficient studies on amphibians in Khorasan Province Iran, this research studies five areas of this province, namely Mashhad, Sarakhs, Bojnoord, Shirvan and Tibad. Totally, 100 samples were collected in these regions from April to November, which were transferred alive to the lab. In the laboratory, the vimnoblastin was injected to the samples proportional to their weights and consequently, the bone marrows were extracted. The obtained solution was dropped from a height of 60 to 70 cm on the frozen slides placed on an inclined surface. Prepared slides were stained and photographed to study the chromosomes. Relative length and arms ratio of the chromosomes were used to recognize the centromere position and chromosomal type. Finally, based on the above studies the related idiograms were derived. This research illustrates that all frogs have a set of 26 2n 26 chromosomes and all toads have a set of 22 chromosomes. It is concluded that all collected frogs and toads from the different stations are Rana ridibunda and Bufo viridis species, respectively. This research shows no sex dimorphism in all samples.