DefinePK

Abstract

Background: Oxidative and osmotic stress cause eryptosis, a type of programmed erythrocyte death known for its phosphatidylserine exposure and cell shrinkage. Sucrose-induced eryptosis is a good model to assess antioxidant treatments because of its oxidative damage. A naturally occurring flavonoid called chrysin has demonstrated encouraging anti-inflammatory and antioxidant capabilities. Objectives: The study of this work aimed to investigate how chrysin protects erythrocytes against hyperosmotic stress and eryptosis brought on by sucrose. The study specifically aimed to evaluate the levels of enzymes, hemolysis inhibition, cell size, and antioxidative activity in erythrocytes under stress. Methods: Human erythrocytes were subjected to varying chrysin and sugar concentrations for 48 hours. Mean cell volume, hemolysis %, hemolysis percentage, oxidative stress indicators (such as catalase and superoxide dismutase activity), and scanning electron microscopy for morphological assessment were all part of the experimental design. ANOVA was used to statistically examine the data. Results: The findings demonstrated that chrysin enhanced antioxidant enzyme activity, maintained cell volume, lowered hemolysis percentage, that result dramatically decreased eryptosis. When compared to stressed cells, SEM demonstrated that erythrocytes treated with chrysin retained their membrane integrity. The findings showed chrysin as an inhibitor for eryptosis. Conclusion: Chrysin's antioxidative properties protect erythrocytes by reducing eryptosis brought on by glucose-induced eryptosis. These findings reveal the protective mechanism of chrysin and suggest as a natural the therapeutic agent to reduce erythrocytes damage by oxidative stress and hyperosmotic shock.