DefinePK

Abstract

Fungi are essential, fascinating and biotechnologically useful group of organisms with an incredible biotechnological potential for industrial exploitation. The knowledge about the overall diversity and uses of fungi is still fragmented and incomplete. Therefore, there are a lot of opportunities to fill the gaps in these areas. Fungi have been used to extract useful enzymes such as Amylase for industrial purposes and their bioactive compounds have shown significant biological properties. The current study was aimed at evaluation of amylase enzyme activity A. oryzae isolated from sugarcane field. Physiochemical analysis was performed to obtain the physiochemical characteristics of the soil. The isolated fungus was identified through microscopy, culture characteristics i.e., hyphal type, spore structure, and spore arrangement and scanning electron microscopy analysis (SEM) analysis. On the basis of these analysis and confirmation of spore structure via SEM analysis the specie was confirmed as Aspergillus oryzae. The amylase enzyme activity was performed through Di-Nitro Salicylic acid (DNSA) method and the protein was quantified through Lowry method of protein quantification. The amylase enzyme extracted from A. oryzae showed enzyme activity of 271.2 U/mg on the fourth day of incubation. It is concluded from current study that A. oryzae has a great potential of producing Amylase enzyme.