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Title: Presence of Anti-Nuclear and Anti-Double-Stranded DNA Antibodies among Healthy Individuals in the Twin Cities (Rawalpindi and Islamabad) of Pakistan
Authors: Muhammad Umer Khan, Amina shafqat, Muhammad Usman Ghani, Saima Younis , Tazeen Zahid, Iram Amin , Muhammad Shahid, Inamullah
Journal: Pakistan Journal of Scientific and Industrial Research (Series B: Biological Sciences)
Publisher: PCSIR Scientific Information Centre
Country: Pakistan
Year: 2025
Volume: 68
Issue: 3
Language: en
Keywords: anti-nuclearanti-double-strandedDNAantibodiesautoimmunity
Background: The study aimed to investigate antinuclear antibodies (ANA) and anti-double- stranded DNA antibodies among healthy individuals in Rawalpindi and Islamabad, emphasizing the need to understand their prevalence in the general population.
Objective: To determine ANA and anti-dsDNA levels, assessing their potential as disease biomarkers in the absence of clinical symptoms among healthy individuals.
Methods: A total of 659 individuals participated in the study, including 503 females (76.3%) and 156 males (23.5%). Blood samples were collected using convenient sampling technique followed by separation of serum using centrifugation. The levels of ANA and anti-dsDNA were quantified using non-competitive ELISA.
Results: The results of the study showed that only 0.91% of individuals tested positive for ANA and 0.46% for anti-dsDNA. This indicates a minimal presence of these antibodies in the healthy cohort. Furthermore, no significant association was found between ANA and anti-dsDNA.
Conclusion: This study highlights a low occurrence of ANA and anti-dsDNA among healthy subjects, suggesting their potential exclusive association with autoimmune diseases.
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