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Abstract

Nanotechnology deals with the use of nanoparticles in various remarkable fields of present time with vast medicinal, agricultural and industrial applications. Biological approaches using microorganisms and plants or plant extracts for metal nanoparticles, have been suggested as valuable alternatives to chemical methods. In present study Syzigium aromaticum L. extracts in water and ethanol were used for the green synthesis of zinc oxide nanoparticles. After being treated with zinc sulphate solution, the color of all the extracts of plant changed indicating the formation of zinc nanoparticles and their color change was also monitored by using Ultraviolet-Visible spectroscopy. Particle size analyzer showed 44.4 nm for water extract nanoparticles and 103nm for ethanolic NPs. Antibacterial activity of these nanoparticles was evaluated against four bacterial strains, namely Klebsiella pneumonia, Pseudomonas aureginosa, Escherichia coli and Acetobactor bauminii. The zinc oxide nanoparticles of S. aromaticum developed by ethanolic extract did not show the significant antibacterial activity against any bacterial strain. The zinc nanoparticles of S. aromaticum developed by water extract showed maximum inhibition zone against K. pneumonia as 1.43mm, P. aureginosa is 1.00mm, E. coli as 2.14mm and against A. bauminii as 2.30mm as compared to 1.15mm, 1.86mm, 1.76mm and 1.53mm respectively by plant extract.
Keywords: Antimicrobial activity; Green nanotechnology; Microwave assisted extraction (MAE); Plant extract; Ultraviolet-visible spectroscopy (UV-Vis); Zinc nanoparticles
http://dx.doi.org/10.19045/bspab.2017.600115