DefinePK

Abstract

Antifungal activity of Syzygium cumini (L.) Skeels bark and leaves were evaluated against Rhizoctonia solani Kuhn, the causal agent of damping-off. Extracts of bark and leaves of S. cumini were prepared in methanol at a concentrations viz. 1%, 2%, 3%, 4% and 5% were tested against the target pathogen. Methanolic bark extract of S. cumini was found more effective and showed high antifungal potential as compared to leaf extract. The methanolic bark extract was subjected to bioassay guided fractionation and different organic fractions like ethyl acetate, chloroform, n-hexane and n-butanol were isolated. The isolated fractions were further serially diluted to check their Minimum Inhibitory Concentration along with a synthetic fungicide Puslan (72% WP). The MIC of various concentrations from (1.56-200 mg mL-1) was recorded for each fraction at the intervals of 24, 48 and 72 hours. n-butanol and Puslan were highly effective in inhibiting the mycelium growth of R. solani with MIC of 1.56 mg/ml.
Keywords: Antifungal activity; Damping off; Jaman; MIC; Plant extract
http://dx.doi.org/10.19045/bspab.2016.50025