DefinePK

Abstract

This exploration was carried out through field inspection in immediate forest neighborhoods of two settlements entitled Killi Tor Shore (K.T.S.) and Killi Shaban (K.S.) in revision region Zarghoon Juniper ecosystem, three districts named Quetta, Sibi and Ziarat, province Balochistan, Pakistan. The leaf samples of Juniperus excelsa trees were collected from these areas and subjected for the DNA isolation. As no genetic analysis is reported for this area Juniper, thus this study is aimed to optimize a protocol for the extraction of DNA. In order to isolate high aggregate of pure and intact DNA would serve great base for the downstream genetic analyses. The impurities of plants predominantly the pine trees are owing to the existence of high magnitude of secondary metabolites, polyphenols and polysaccharides. After the application of different acknowledged protocols for the DNA extraction of Juniperus excelsa trees it was perceived none of the protocols extracted pure and high yield DNA. Therefore, with the adaptation in technique an optimized protocol abetted in isolating pure DNA chiefly for tree Juniperus excelsa. This optimized protocol applied multiple times consequently made possible the isolation of pure DNA of Juniper.
Keywords: Ziarat Juniper; DNA extraction; Secondary metabolites
http://dx.doi.org/10.19045/bspab.2015.44026