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Title: 01. Studies on genetic diversity of cotton using RAPD markers
Authors: Shazia Anwer Bukhari, Muhammad Ahsan Iqbal, Shamila Naz, Mahmood-ur-Rahman
Journal: Pure and Applied Biology (PAB)
Publisher: Bolan Society for Pure and Applied Biology
Country: Pakistan
Year: 2021
Volume: 3
Issue: 3
Language: en
The study was conducted to assess the genetic diversity in 12 cotton varieties (Gossypium hirsutum). Diverse genepool is significantly important in variety development programs and assessment of genetic variability is the first stepin any crop improvement programs. The results showed considerable variability in the genotypes under study. FourRAPD primer series (A, C, J & K) were used in this study. Most of the primers used showed significantpolymorphism. The genotypes were clustered into four groups (A, B, C and D). Four groups contained cluster andsub clusters which showed the similarity and dissimilarity of the genotypes. In group A, there were four genotypes,FH-169, FH-170, FH-172 and FH-113 while in group B, FH-941, CIM-241, CRIS-134 and PB-900 were present. Ingroup C and D, there were three varieties i.e. CIM-XCP 29, CIM-506, CIM-448 and one genotype (CIM-240)respectively. This information would be useful for cotton breeding programs and in the hybridization experiments.
Keywords: Cotton, Genetic Diversity, PCR, RAPD Markers
http://dx.doi.org/10.19045/bspab.2014.33002
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