DefinePK

Abstract

Probiotics is widely used in aquaculture. Aquaculture is the controlled cultivation of aquatic organisms in a range of freshwater and marine environments. Present study examined the impact of dietary supplementation of probiotics (Bacillus Subtilis, Lactobacillus plantarum), yeast (Saccharomyces cerevisiae) and digestive enzymes (Amylase, Protease, Lipase & Pancreatin) on the antioxidant status and hematological analysis of grass carp reared in glass aquaria at the Aquaculture and Fisheries Laboratory, Department of Zoology, Wildlife and Fisheries, PMAS-Arid Agriculture University Rawalpindi, Pakistan. A total of 90 fingerlings of grass carp were reared in the laboratory, having the same environmental conditions. The fish were fed in different groups viz; Control group (C) were fed with commercially available supplementary fish diet while group one (T1) and group two (T2) were fed with Supplementary feed with addition of 2g/kg and 4g/kg consortium of probiotics, yeast and digestive enzymes respectively. The growth parameters were checked on a fortnightly basis to adjust the quantity of feed given. At the end of the experiment (90 days), random sampling was done from each group to analyze antioxidant status and hematological profile. The Results show that supplementary diets with addition of probiotics, yeast and digestive enzymes enhanced the antioxidant status in group 1 (50.092 µg/mL); Hematological parameters i.e., Red Blood Cells (RBC), were observed significantly higher in T2 group (3.10 x105/µL) than T1 and Control group, while WBC; white blood cells (9.94 ×104/µL) and Hemoglobin (Hb)were significantly higher in T1 group (8.30 g/dL) than T2 and Control group (P <0.05). The results of the present study suggest the addition of consortium of probiotics, yeast and digestive enzymes in fish feed can improve the health status of grass carp. Such component used as feed additives are significant in achieving a sustainable and efficient fish production.