DefinePK hosts the largest index of Pakistani journals, research articles, news headlines, and videos. It also offers chapter-level book search.
Title: Anti-diabetic activity of Carica Papaya Linn in Alloxan-Induced diabetic rats.
Authors: Jaweriya Fazal, Lubna Naz, Sumaira Sohail, Ghazala Yasmeen, Nazish Iqbal Khan, Nazneen Zehra
Journal: International Journal of Endorsing Health Science Research
Publisher: Advance Educational Institute & Research Centre
Country: Pakistan
Year: 2022
Volume: 10
Issue: 1
Language: English
Keywords: Diabetes MellitusAlloxanCarica Papaya LinnAntioxidant.
Background: Type I Diabetes Mellitus (TIDM) is a disease known to occur in any age group, but most of the people affected are diagnosed in their mid-teenage. It is marked by hyperglycemia due to autoimmune destruction of insulin-producing pancreatic beta cells. Herbal treatment of DM has attracted attention in the last few decades because of its ability to improve diabetic complications such as nephrotic deterioration and oxidative stress. The present research is sought to determine the anti-diabetic potential of Carica Papaya leaf extract in Alloxan-induced diabetic rats.
Methodology: For this study, eighteen healthy male Wistar rats weighing 150-200 g were made into three groups (n=6), two of which (Group II and Group III) were induced DM through Alloxan, whereas control (Group I) remained untreated. Group III diabetic rats were treated with 250 mg/kg body weight of papaya leaf extract after confirming hyperglycemia. Animals were sacrificed on the 22nd day for biochemical analysis.
Results: This study showed that papaya leaf extract administration caused a significant reduction in the levels of plasma glucose, AST, ALP, ALT, serum urea, blood urea nitrogen (BUN), and creatinine in the papaya-treated group as compared to the Alloxan-treated group. It also increased plasma insulin levels compared to the diabetic-control (Alloxan-treated) group. Activity and concentration of major antioxidant enzymes such as CAT, SOD, and GSH in treated animals were also significantly increased compared to the diabetic (Alloxan-treated) group.  
Conclusion: Based on the findings, it is concluded that the C. Papaya leaf extract exhibits antidiabetic and antioxidative potential against alloxan-induced TIDM.
To investigate the anti-diabetic, hepatoprotective, and nephroprotective effects of Carica papaya leaf extract in Alloxan-induced diabetic rats.
Eighteen male Wistar rats were divided into three groups (n=6). Group I was the untreated control. Groups II and III were induced with Type I Diabetes Mellitus (TIDM) using Alloxan. Group III received 250 mg/kg body weight of Carica papaya leaf extract (CPLE) after hyperglycemia was confirmed. Rats were sacrificed on the 22nd day for biochemical analysis. Biochemical parameters assessed included plasma glucose, insulin, liver enzymes (AST, ALT, ALP), kidney function markers (Urea, Creatinine, BUN, Total Protein), and antioxidant enzymes (CAT, SOD, GSH), as well as lipid peroxidation (MDA). Statistical analysis was performed using SPSS version 16.0 with an independent T-test, with significance set at p < 0.05.
graph TD
A[Animal Selection & Acclimatization] --> B[Study Design: Group Assignment];
B --> C[Diabetes Induction with Alloxan];
C --> D[Confirmation of Hyperglycemia];
D --> E[CPLE Administration Group III];
E --> F[Sample Collection Blood & Organs];
F --> G[Homogenate Preparation];
G --> H[Biochemical Analysis];
H --> I[Statistical Analysis];
I --> J[Results Interpretation];
J --> K[Discussion & Conclusion];
Alloxan induces diabetes by damaging pancreatic beta cells through ROS production and inhibiting glucokinase. Carica papaya leaf extract demonstrated anti-diabetic effects by potentially reducing glucose absorption, increasing peripheral glucose utilization, activating remaining beta cells, and regenerating them. The extract also exhibited hepatoprotective and nephroprotective effects, evidenced by the normalization of liver enzymes and kidney function markers. The observed antioxidant activity is attributed to the bioactive components in the extract, which scavenge free radicals and boost endogenous antioxidant enzymes.
Administration of Carica papaya leaf extract (CPLE) significantly reduced plasma glucose, AST, ALP, ALT, serum creatinine, BUN, and urea levels in diabetic rats compared to the Alloxan-treated group. CPLE also significantly increased plasma insulin levels and the activity and concentration of antioxidant enzymes (CAT, SOD, GSH). Lipid peroxidation (MDA) levels were significantly reduced in the CPLE-treated group. Body weight also showed a significant increase in the CPLE-treated group compared to the diabetic group.
Carica papaya leaf extract possesses significant anti-diabetic and antioxidative properties against alloxan-induced TIDM in rats. The leaves can be a valuable source for pharmaceutical agents, though further research is needed to elucidate specific phytochemical components and their exact mechanisms of action.
1. Alloxan dose: Alloxan was administered intraperitoneally at approximately 120 mg/kg to induce diabetes.
2. CPLE dosage: Carica papaya leaf extract was administered at 250 mg/kg body weight.
3. Study duration: Rats were sacrificed on the 22nd day of the experiment.
Loading PDF...
Loading Statistics...