PREVALENCE OF MASTITIS IN NILI RAVI BUFFALO HERD AT LIVESTOCK EXPERIMENT STATION BHUNIKEY, PATTOKI

Article Information

Title: PREVALENCE OF MASTITIS IN NILI RAVI BUFFALO HERD AT LIVESTOCK EXPERIMENT STATION BHUNIKEY, PATTOKI

Authors: I. Anwar

Journal: Pakistan Journal of Science (PJS)

HEC Recognition History

Category	From	To
Y	2024-10-01	2025-12-31
Y	2023-07-01	2024-09-30
Y	2022-07-01	2023-06-30
Y	2020-07-01	2021-06-30
Y	1900-01-01	2005-06-30

Publisher: Advance Educational Institute & Research Centre

Country: Pakistan

Year: 2022

Volume: 74

Issue: 2

Language: English

DOI: 10.57041/pjs.v74i2.773

Keywords: Antibiotic sensitivityCalifornia Mastitis TestEnrofloxacinGentamicinMastitisNili-Ravi buffalo.

Abstract

Mastitis is one of the major and keen parameter for quality and quantity of milkproduction. The recent study was conducted to estimate the occurrence rate of mastitis in four differentseasons of the year and its therapeutic cure in Llivestock Experiment Station Nili Ravi Bhunikey. Forthis purpose, the total of 1188 milk samples were screened for mastitis through California MastitisTest. Out of total 1188 milk samples, the prevalence rate of mastitis was 9.7% (27/279) in winter, 14%(40/286) in spring , 7.2% (23/320) in summer and 6% (18/303) in autumn respectively. An overallprevalence rate of mastitis was 9 %( 108/1188).The screened positive samples were subjected toculture on culture media. Seven antibiotics were selected for the fortitude of antibiotic sensitivityagainst the cultured organisms. Antibiotics used in current research were Gentamicin, Enrofloxacin,Amoxycillin, Norfloxacin, Tylosin, Oxytetracycline and Chloramphenicol. The efficacy of thedifferent antibiotics was checked by the configuration of the zone of inhibition. The outcome showedthat the incidence of mastitis was greater in spring and lower in autumn. From selected antibiotics,Enrofloxacin and Gentamycine have resulted in more effective against the cultured organism.

Disclaimer: The following sections are produced through an intensive multi-stage analytical pipeline developed by DefinePK, utilizing advanced natural-language processing, domain-specific knowledge models, and automated cross-referencing systems. Despite the rigor and computational effort involved, the generated summary may still contain inaccuracies or incomplete interpretations. Users are strongly advised to verify all critical information against the original paper and its authoritative sources.

Research Objective

To estimate the occurrence rate of mastitis in Nili Ravi buffaloes across four different seasons and to determine the therapeutic cure through antibiotic sensitivity testing.

Methodology

A one-year study (June 2020 to May 2021) divided into four seasons. A total of 1188 milk samples were collected aseptically from Nili Ravi buffaloes. Samples were screened for mastitis using the California Mastitis Test. Positive samples were cultured, and antimicrobial susceptibility testing was performed using the disc diffusion method on Mueller-Hinton agar against seven common veterinary antibiotics: Gentamicin, Enrofloxacin, Amoxycillin, Norfloxacin, Tylosin, Oxytetracycline, and Chloramphenicol. Data was analyzed statistically using percentages in Excel sheets.

Methodology Flowchart

                        graph TD;
    A[Collect Milk Samples 1188] --> B[Screen with California Mastitis Test];
    B --> C[Positive Samples?];
    C -- Yes --> D[Culture on Media];
    C -- No --> E[Discard];
    D --> F[Antibiotic Sensitivity Testing];
    F --> G[Analyze Results];
    G --> H[Determine Prevalence and Effective Antibiotics];

Discussion

The study's findings on mastitis prevalence align with some previous research, while seasonal variations in incidence are noted. The effectiveness of Enrofloxacin and Gentamicin against mastitis-causing organisms is highlighted, with comparisons made to other studies regarding antibiotic sensitivity.

Key Findings

The overall prevalence rate of mastitis was 9% (108/1188). Seasonal prevalence was highest in spring (13.9%) and lowest in autumn (5.94%). Enrofloxacin and Gentamicin were found to be the most effective antibiotics against the cultured organisms, showing the highest sensitivity.

Conclusion

Mastitis occurs more frequently in spring and less in autumn in Nili Ravi buffaloes, likely due to temperature influencing bacterial growth. Enrofloxacin and Gentamicin are identified as the most effective drugs for in vitro treatment against cultured mastitogens.

Fact Check

- Total milk samples screened: 1188
- Overall mastitis prevalence: 9% (108/1188)
- Highest seasonal prevalence: Spring (13.9%)

Mind Map

Loading PDF...

Loading Statistics...

DefinePK

Select Collection