DefinePK

Abstract

Objective: The current study was designed to examine the antibacterial activity of various extracts of Citrullus colocynthis fruits against Helicobacter pylori, Salmonella enterica, Shigella flexneri, Campylobacter jejuni, Micoplasma pneumonia, and Shigella dysenteriae.Methodology: Antimicrobial activity was evaluated by agar disc diffusion method. The available genome of C. colocynthis was utilized to retrieve sequence of highly conserved genes matK, rpl20, rpl32, and rps7 and to identify their relevant genes in C. rehmii.Results and Conclusion: The maximum antimicrobial activity was shown by ethanol extract (S. f. 32mm, C. j. 27mm, H. p. 26mm, S. d. 22mm, M. p. 21mm, S. e. 19mm) while least activity was given by n-hexane extract (S. f. 13mm, C. j. 11mm, H. p. 18mm, S. d. 8mm, M. p. 10mm, S. e. 8mm) when compared with standard antibiotic levofloxacin (S. f. 30mm, C. j. 30mm, H. p. 30mm, S. d. 28mm, M. p. 25mm, S. e. 20mm). All extracts had given maximum response against H. pylori and S. flexneri and least response against M. pneumoniae and S. enterica. The in-silico genome-wide investigation studies showed that all four genes of C. rehmii were identified with high conservation based on motif distribution and phylogenetic relations.