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Abstract

ABSTRACT In order to investigate the anti-aging effects of Lycium ruthenicum Murr. granules (HGQ) and potential underlying mechanism of actions, Caenorhabditis elegans was exploited as model organisms. The anti-aging capacity of HGQwas determined by lifespan and lipofuscin fluorescence assays in wild-type C. elegans N2. Calorie restriction pathway was detected by pharyngeal pumping rate assay and lifespan examination in sir-2.1 mutant nematodes. The antioxidant activity of HGQ was investigated by determining reactive oxygen species (ROS) level, paraquat-stress survival rate, malondialdehyde (MDA) content as well as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities.We observed that HGQ exerted little effect on the growth of wild-type N2 nematodes at concentration < 0.6 mg/ml, and 0.05 mg/ml of the HGQ significantly prolonged the lifespan and reduced the lipofuscin level in N2 nematodes. However, the HGQ failed to influence the pharyngeal pumping rate in N2 nematodes or to affect the lifespan of sir-2.1 mutant nematode VC199, indicating the anti-aging effect of HGQ is independent of calorie restriction pathway. Further experiments showed that the HGQ decreased ROS and MDA contents and increased the survival rate and SOD (not CAT or GPx) activities in paraquat-stressed N2 nematodes. Our results suggest that HGQcan effectively delay the senescence of C. elegans, and this effect may be related with its significant antioxidant activity.