DAILY VARIATION OF BRAIN PROTEIN IN THE PRE-FRONTAL CORTEX OF ARVICANTHIS ANSORGEI

Article Information

Title: DAILY VARIATION OF BRAIN PROTEIN IN THE PRE-FRONTAL CORTEX OF ARVICANTHIS ANSORGEI

Authors: Nauman Aziz, Tahir Maqbool, Amer Shoaib, Mahwish Saeed, Shumaila Doggar, Hafiza Swaiba Afzal

Journal: Pakistan Armed Forces Medical Journal (PAFMJ)

HEC Recognition History

Category	From	To
Y	2024-10-01	2025-12-31
Y	2023-07-01	2024-09-30
Y	2021-07-01	2022-06-30
Y	2020-07-01	2021-06-30
Y	1900-01-01	2005-06-30

Publisher: Army Medical College, Rawalpindi.

Country: Pakistan

Year: 2023

Volume: 73

Issue: Supplementary 1

Language: English

DOI: 10.51253/pafmj.v73iSUPPL-1.12978

Keywords: Pre-frontal cortexZeitgeber Time (ZT)Circadian rhythmstotal proteinBradford assayArvicanthis ansorgei.

Abstract

Objective: To examine the daily variation of total protein contents in the pre-frontal cortex of the diurnal rodents, Arvicanthis&nbsp;ansorgei.Study Design: Quasi-experimental study.&nbsp;Place and Duration of Study: Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore Pakistan, form&nbsp;Jan to May 2023.&nbsp;Methodology: Male young rats were forfeited at various zeitgeber times (ZT1, ZT5, ZT9, ZT13, ZT17, and ZT21), ZT0, and ZT12characterizing lights on and off respectively. The brains were separated on ice and the pre-frontal cortex was isolated. The&nbsp;quantity of total protein contents was assessed using the Bradford test on the brain supernatants.&nbsp;Results: The total brain protein contents in the prefrontal cortex of Arvicanthis ansorgei follow a circadian variation during&nbsp;the 24 hours of the day. Repeated measure ANOVA indicated a significant difference among all the six different time points,&nbsp;F (1,5)=595.476, p&lt;0.001.Conclusion: The first evidence that total protein contents alter at different times of the day in the pre-frontal cortex with a peakat ZT17 (5h after light off) and low at ZT1 (1h after light on) in diurnal rodents.&nbsp;

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Research Objective

To examine the daily variation of total protein contents in the pre-frontal cortex of the diurnal rodents, Arvicanthis ansorgei.

Methodology

Quasi-experimental study. Male young rats (Arvicanthis ansorgei) were acclimatized to a 12:12 light-dark cycle. They were divided into six groups and sacrificed at various zeitgeber times (ZT1, ZT5, ZT9, ZT13, ZT17, and ZT21). Brains were separated, pre-frontal cortex isolated, and total protein content assessed using the Bradford test on brain supernatants.

Methodology Flowchart

                        graph TD;
    A["Acclimatize Rats to Light-Dark Cycle"] --> B["Divide Rats into 6 Groups by Zeitgeber Time"];
    B --> C["Sacrifice Rats at Specific ZT"];
    C --> D["Isolate Pre-frontal Cortex"];
    D --> E["Prepare Brain Supernatants"];
    E --> F["Quantify Total Protein using Bradford Assay"];
    F --> G["Analyze Data using Repeated Measure ANOVA"];
    G --> H["Determine Daily Protein Variation"];

Discussion

The study provides the first evidence of daily variation in total protein content in the pre-frontal cortex of Arvicanthis ansorgei, a diurnal rodent. This variation follows a circadian pattern, with a peak during the dark phase (ZT17) and a trough during the light phase (ZT1). This finding contrasts with some studies on specific proteins that show higher levels during the light period. The observed rhythmicity may be important for normal physiological processes and cognitive functions.

Key Findings

Total brain protein content in the prefrontal cortex of Arvicanthis ansorgei follows a circadian variation. A significant difference was found among the six time points (F(1,5)=595.476, p<0.001). The peak protein content was observed at ZT17 (5 hours after light off), and the lowest at ZT1 (1 hour after light on).

Conclusion

Total protein content in the Arvicanthis ansorgei pre-frontal cortex exhibits a circadian pattern throughout the 24-hour day, with peak levels at ZT17 and lowest levels at ZT1.

Fact Check

1. Study Duration: The study was conducted from January to May 2023. (Confirmed by "Place and Duration of Study: Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore Pakistan, form Jan to May 2023.")
2. Statistical Significance: Repeated measure ANOVA indicated a significant difference among the six time points (p<0.001). (Confirmed by "Repeated measure ANOVA indicated a significant difference among all the six different time points, F (1,5)=595.476, p<0.001.")
3. Peak Protein Time: The peak of total protein content was observed at ZT17. (Confirmed by "The peak of the total protein contents was observed at ZT17 (5 hours after lighting off)")

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