DefinePK

Abstract

Optimization parameter for production several xylanases have already been studied, but only a few derived from marine bacteria especially Bacillus sp. Focus on this research is optimation of process parameters for xylanase production and scale-up production in stirred tank bioreactor using corn cob raw biomass as a substrate. Maximum xylanase production was obtained at 1,5% corn cob concentration, pH medium 6, temperature fermentation 30C, and agitation rate 200 rpm. For production using agricultural waste, production with corn cob (2,963 U/mL) is higher than rice straw (1,938 U/mL) and sugarcane bagasse (1,728 U/mL). Optimization process parameters increased xylanase activity from 1,864 U/mL at 96 h to 2,963 U/mL at 24 h incubation. The scale-up of the fermentation process up to 2 L stirred tank bioreactor with the optimum condition at flask scale, significantly increased xylanase activity to 3,519 U/mL.