DefinePK

Abstract

Abstract | This study was designed to compare the efficient production of ethanol in separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) process. Fungal strains of Saccharomyces cervisae, Trichoderma viride, Trichoderma koninji and Trichoderma harzianum was used for subsequent process using saw dust as substrate. Three major processes convert lignocelluloses to bioethanol i.e. pretreatment of biomass, enzymatic hydrolysis of raw material and fermentation. The sawdust was pretreated with 2.5% NaOH and further processed for SHF and SSF. Maximum saccharification was observed by T. viride (10.17%) followed by T. harzianum (9.19%) and T. koninji (6.91%) at 35˚C for 48h. Among both strategies, SSF combination of T. viride and S. cerevisiae gave maximum ethanol production (3.88%) after 12 days of fermentation at 30˚C.