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Enzymatic Production of N-Acetyl-D-Glucosamine from Chitin Using Crude Enzyme Preparation of Aeromonas sp. PTCC1691

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Title: Enzymatic Production of N-Acetyl-D-Glucosamine from Chitin Using Crude Enzyme Preparation of Aeromonas sp. PTCC1691

Authors: K. Jamialahmadi, J. Behravan, M. Fathi Najafi, M. Tabatabaei Yazdi, A. R. Shahverdi, M. A. Faramarzi

Journal: Biotechnology

HEC Recognition History
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Publisher: Asian Network for Scientific Information (ANSInet)

Country: Pakistan

Year: 2011

Volume: 10

Issue: 3

Language: English

DOI: 10.10.3923/biotech.2011.292.297

Keywords: Enzymatic hydrolysisChitinAeromonas sp. PTCC 1691crude enzymeN-acetyl-D-glucosamine

Categories
Food Science (9432) Microbiology (14542) Biochemistry (18816) Biotechnology (6089) Enzyme (11)

Abstract

The present study was directed to the production of N-acetyl-D-glucosamine (GlcNAc) by partial hydrolysis of colloidal α-chitin using the crude enzyme preparation of Aeromonas sp. PTCC 1691. The selective production of GlcNAc was observed during continuous hydrolysis of chitin and at the same time period, the production of GlcNAc oligomers was negligible (below 0.5%). The maximum production of GlcNAc, a yield of 79%, was obtained after 24 h of incubation at the enzyme-substrate ratio of 2 U mg-1. These results suggest that the multi-chitinolytic enzyme complex produced by Aeromonas sp. PTCC 1691 could be useful for bioconversion of chitin waste into N-acetyl-D-glucosamine for industrial applications.

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