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Abstract

ABSTRACT


The present study was carried out to optimized medium
composition and culture conditions to enhance the bacteriocin production
from Lactococcus lactis CCSULAC1 in MRS medium. The optimum temperature
and initial pH for bacteriocin production was 7.5 and 35C,
respectively. Lactococcus lactis CCSULAC1 displayed the highest
bacteriocin activity when grown in modified MRS medium containing soya
extract SEMRS as nitrogen source instead of other selective constituents.
Different culture media including MRS with 1.5 tween, TGEA, TGYA, Elliker146s
media were also studied for bacteriocin production. The bacteriocin showed
broad antimicrobial spectrum determined by well diffusion assay against
E. coli, Enterobacter sp., S. aureus, Pseudomonas
sp., B. polymyxa, B. subtilis, S. typhii, Micrococcus
sp. but shown no effect on Candida albicans, Shigella sp.,
Klebsiella sp. and S. paratyphii. Replacement of soya extract
with yeast extract demonstrated the better yield of bacteriocin. The modified
SEMRS is a cost effective medium that can be helpful for large scale industries.

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