DefinePK

Abstract

ABSTRACT


In the present study, attempts have been made in optimising the conditions for whey lactose hydrolysis to prepare whey based soups or syrups. -galactosidase enzyme was extracted from Kluyveromyces marxianus and partially purified. Two different alkali Potassium hydroxide KOH and Sodium hydroxide NaOH were used to bring the whey to required pH. Then, the whey lactose hydrolysis was carried out at two different temperatures, 25 and 37C using whey with different pH 6.6 and 7.0. Out of eight combinations of alkali metal ions K, Na, pH 6.6 and 7.0 and temperature 25 and 37C used, yielded increased lactose hydrolysis than that of Na ions. pH 7.0 was found to be effective in lactose hydrolysis than pH 6.6. Hydrolysis incubation temperature 37C showed more hydrolysis percentage than 25C. From the above results, it may be concluded that, pH 7.0, temperature 37C and alkali metal ion K combination yielded the possible highest lactose hydrolysis percentage 86.8.

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