DefinePK

Abstract

Small crab by-product such as non-crab shells waste is a potential source of proteins and amino acids. Therefore, this research was to examine the non-shell small crab amino acid and determine the effect of hydrolysate processing on amino acid levels. The study used crab (Portunus Pelagicus) lemi as part of the non-shell crab waste. Lemi was converted into hydrolysate using the alcalase enzyme. Amino acid profile was analyzed before and after the hydrolysis process. UHPLC (Ultra-High-Performance Liquid Chromatography) was used to determine the level of amino acids level. The use of hydrolysis technology increased the total of amino acids by 45.83%, from 21.32 g.100 g-1 to 31.09 g.100 g-1. The percentage of essential amino acids compared to non-essential amino acids is 58.06% of the total amino acid from the hydrolysate of a non-shell small crab waste. The hydrolysis technology also increases the chemical score. The amino acid histidine had a high chemical score after the hydrolysis process. The study suggested that by-products derived from small crab waste can be considered essential sources of proteins and amino acids for malnutrition disease.