DefinePK

Abstract

Ever increasing biotechnological industries require an enlarged survey of microorganisms which might be useful for industries. In the present work optimization parameters for alpha amylase production by Penicillium notatum IBGE 03 in submerged fermentation were studied. Various agricultural based by-products (sunflower waste, cotton stalk, rice husk, date syrup and molasses) were used as sources of carbon. Optimal conditions for the production of α-amylase (6.58 U/mL) by P. notatum IBGE 03 were observed when the strain was grown on culture medium M1 containing corn steep liquor as a source of nitrogen, molasses as a source of carbon after 48 h of incubation at 30° C, initial pH 5.5, inoculum size of 5x106 conidia in 50 mL of culture medium and agitation rate of 150 rev/min. The strain was proved thermo (up to 60° C) and pH (up to 9.0) stable so it might be a potential strain for industrial utilization.
Keywords: Alpha-amylase; Penicillium notatum IBGE 03; Optimization
http://dx.doi.org/10.19045/bspab.2015.43005