DefinePK

Abstract

The study of the phytochemical composition and antioxidant activity of four green tea varieties from the NTHRI Shinkiari, Pakistan were selected (NTHRI-I, NTHRI-II, Rustam Khan, and Abdul Waheed) for analysis. Tea leaves were first plucked then processed through various steps. Standard methods were carried out to analyze proximate composition of moisture and ash content. The moisture contents of tea samples Abdul Waheed, Rustam Khan, NTHRI-II and NTHRI-I were 0.33%, 6.8%, 10.00   % and 15.3% respectively. Tea samples were evaluated and analyzed for chemical parameters i.e. polyphenols, caffeine and anti-oxidant analysis, the cumulative antioxidant activity was validated by assessing the free radical scavenging ability of all samples. The findings showed that the IC50 values ranged from 1.43 to 5.55 μg/mL. It was also discovered that the level of caffeine varied between 2.06% and 2.58%, depending on the four selected varieties of green tea. The average polyphenol content in different green tea varieties which is locally produce at NTHRI Shinkiari, Mansehra i.e. Rustam Khan, Abdul Waheed, NTHRI-II and NTHRI-II is 1.25896mg/ml, 1.6765mg/ml, 1.62333mg/ml and 1.2623mg/ml respectively in the levels of these important antioxidant compounds. For qualitative analysis of green tea samples extracts of different solvents were used i.e. distilled water and methanol. The results indicated that all the four varieties contain phytochemicals (tannins, saponin, alkaloids, flavonoids, phenols, terpenoid and glycosides). This study contributed to the understanding of the potential health benefits and physicochemical profile of green tea produced in Pakistan, which may have been useful for the Pakistani tea industry in fostering the production and use of Pakistani-origin green tea for health benefits.